DHFR Polyklonaler Antikörper

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DHFR Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15194-1-PBS

Synonyme

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  WB analysis using 15194-1-AP (same clone as 15194-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15194-1-AP (same clone as 15194-1-PBS)

  WB result of DHFR antibody (15194-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHFR transfected HeLa cells. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 15194-1-AP (same clone as 15194-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15194-1-AP (same clone as 15194-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 15194-1-AP (same clone as 15194-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 15194-1-AP (same clone as 15194-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Raji using 15194-1-AP (same clone as 15194-1-PBS)

  IP result of anti-DHFR (IP:15194-1-AP, 3ug; Detection:15194-1-AP 1:500) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15194-1-AP (same clone as 15194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15194-1-AP (same clone as 15194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15194-1-AP (same clone as 15194-1-PBS)

  Immunofluorescent analysis of HeLa cells using 15194-1-AP (DHFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15194-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DHFR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHFR fusion protein Ag7331
• Vollständiger Name: dihydrofolate reductase
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000192
• Gene symbol: DHFR
• Gene ID (NCBI): 1719
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).