DHH Polyklonaler Antikörper

DHH Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13889-1-PBS

Synonyme

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• 

  WB analysis of mouse skeletal muscle using 13889-1-AP (same clone as 13889-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13889-1-AP (DHH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13889-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 13889-1-AP (same clone as 13889-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13889-1-AP (DHH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13889-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13889-1-PBS bindet in WB, Indirect ELISA DHH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHH fusion protein Ag4850
• Vollständiger Name: desert hedgehog homolog (Drosophila)
• Berechnetes Molekulargewicht: 396 aa, 44 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC033507
• Gene symbol: DHH
• Gene ID (NCBI): 50846
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Desert hedgehog (DHH) is one of three ligands in the hedgehog signaling pathway that controls gonad 

morphogenesis and nerve perineurium differentiation.

What is the molecular weight of DHH? Is DHH post-translationally modified?

DHH protein is synthesized in a precursor form of ~45 kDa. Hedgehog proteins contain a small N-terminal signal 

peptide, which is cleaved off intracellularly, leading to 39 kDa species. Hedgehog proteins are then subject to 

autoproteolysis, which leads to the generation of a 19 kDa N-terminal fragment with added cholesterol moiety 

and a 25 kDa C-terminal fragment (PMID: 7985023). The C-terminal fragment catalyzes the precursor proteolysis, 

while the N-terminal fragment gets secreted from cells and acts as an active ligand in the hedgehog signaling 

pathway.

What is the subcellular localization of DHH?

DHH protein is subject to autoproteolysis, as described above, and the N-terminal fragment is secreted from 

cells in a regulated manner. The amounts of extracellular hedgehog proteins determine local and long-range 

signaling (PMID: 7736596 and 7885476).

What is the tissue expression pattern of DHH?

Unlike other hedgehog ligands, expression of DHH is very tissue-specific. DHH is strongly expressed in Steroli 

cell precursors, adult sterol cells, and in Leydig cells in testis (PMID: 8805249, 12050120) but also in granulosa 

cells in ovaries (PMID: 15878962), as well as in Schwann cells-glia of the peripheral nervous system 

(PMID: 7589793).

What is the role of DHH in morphogenesis?

DHH plays a role in the correct development of male gonads and perineurium. Loss of DHH leads to peripheral 

neuropathy and gonadal dysgenesis (PMID: 11017805).