DHODH Monoklonaler Antikörper

DHODH Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2B2C6

Kat-Nr. : 67977-1-Ig

Synonyme

DHOdehase, DHODH, dihydroorotate dehydrogenase, Dihydroorotate oxidase

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  WB analysis of LNCaP using 67977-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67977-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67977-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67977-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67977-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67977-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67977-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

67977-1-Ig bindet in WB, ELISA DHODH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DHODH fusion protein Ag6649
• Vollständiger Name: dihydroorotate dehydrogenase
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC065245
• Gene symbol: DHODH
• Gene ID (NCBI): 1723
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

Publikationen

Species	Application	Title
monkey	WB	Vet Res; Vidofludimus inhibits porcine reproductive and respiratory syndrome virus infection by targeting dihydroorotate dehydrogenase; Authors - Yuanqi Yang
mouse	WB	ACS Nano; Engineering Biodegradable Hollow Silica/Iron Composite Nanozymes for Breast Tumor Treatment through Activation of the "Ferroptosis Storm"; Authors - Panpan Xue
human,rat	WB	Front Pharmacol; Combination decoction of Astragalus mongholicus and Salvia miltiorrhiza mitigates pressure-overload cardiac dysfunction by inhibiting multiple ferroptosis pathways; Authors - Qiyao Xu