DHRS11 Polyklonaler Antikörper

DHRS11 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 20172-1-AP

Synonyme

ARPG836, DHRS11, SDR24C1

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  WB analysis of mouse liver using 20172-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20172-1-AP (DHRS11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20172-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20172-1-AP (DHRS11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 20172-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20172-1-AP (DHRS11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 20172-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20172-1-AP (DHRS11 Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human breast cancer using 20172-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20172-1-AP (DHRS11 Antibody) at dilution of 1:50(under 40x lens).

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  IHC staining of mouse small intestine using 20172-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20172-1-AP (DHRS11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 20172-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20172-1-AP (DHRS11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, COLO 320-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: Maus-Dünndarmgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

20172-1-AP bindet in WB, IHC, ELISA DHRS11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHRS11 fusion protein Ag14005
• Vollständiger Name: dehydrogenase/reductase (SDR family) member 11
• Berechnetes Molekulargewicht: 260 aa, 28 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC002731
• Gene symbol: DHRS11
• Gene ID (NCBI): 79154
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Exp Clin Cancer Res; Pericytes recruited by CCL28 promote vascular normalization after anti-angiogenesis therapy through RA/RXRA/ANGPT1 pathway in lung adenocarcinoma; Authors - Ying Chen