DHRS4 Polyklonaler Antikörper

DHRS4 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15279-1-PBS

Synonyme

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  WB analysis of rat liver using 15279-1-AP (same clone as 15279-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15279-1-AP (same clone as 15279-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 15279-1-AP (same clone as 15279-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15279-1-AP (same clone as 15279-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 15279-1-AP (same clone as 15279-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 15279-1-AP (same clone as 15279-1-PBS)

  IP result of anti-DHRS4 (IP:15279-1-AP, 4ug; Detection:15279-1-AP 1:400) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 15279-1-AP (same clone as 15279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 15279-1-AP (same clone as 15279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervix using 15279-1-AP (same clone as 15279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervix using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervix using 15279-1-AP (same clone as 15279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervix using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15279-1-PBS bindet in WB, IHC, IP, Indirect ELISA DHRS4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHRS4 fusion protein Ag7398
• Vollständiger Name: dehydrogenase/reductase (SDR family) member 4
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC003019
• Gene symbol: DHRS4
• Gene ID (NCBI): 10901
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHRS4(Dehydrogenase/reductase SDR family member 4) is also named as PHCR , NRDR ,PSCD , SCAD-SRL, and belongs to the short-chain dehydrogenases/reductases (SDR) family. It can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Human DHRS4 is a tetramer composed of 27 kDa subunits, which is inactivated at low temperature without dissociation into subunits(PMID:18571493).