DHRS9 Polyklonaler Antikörper

DHRS9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 14560-1-AP

Synonyme

EC:1.1.1.209, EC:1.1.1.105, 3-alpha-HSD, 3-alpha hydroxysteroid dehydrogenase, 3ALPHA HSD

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  WB analysis using 14560-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14560-1-AP (DHRS9 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14560-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14560-1-AP (DHRS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse trachea using 14560-1-AP

  mouse trachea tissue were subjected to SDS PAGE followed by western blot with 14560-1-AP (DHRS9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 14560-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14560-1-AP (DHRS9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14560-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14560-1-AP (DHRS9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 14560-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using DHRS9 antibody (14560-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 14560-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 14560-1-AP (DHRS9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, HT-29-Zellen, Maus-Tracheagewebe, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14560-1-AP bindet in WB, IHC, IF/ICC, ELISA DHRS9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHRS9 fusion protein Ag6116
• Vollständiger Name: dehydrogenase/reductase (SDR family) member 9
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC058883
• Gene symbol: DHRS9
• Gene ID (NCBI): 10170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dehydrogenase/reductase 9(DHRS9), is a member of the short-chain dehydrogenases/reductases (SDR) family. DHRS9 has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. DHRS9 has 4 isoforms with molecular weight of 19, 30, 35, 42 kDa, and demonstrates oxidoreductase activity toward hydroxysteroids. DHRS9 is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus.

Publikationen

Species	Application	Title
human	WB	Front Mol Biosci; Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype.; Authors - Shuang Bai
human,mouse	IF,WB	EPMA J; Identifying potential signatures for atherosclerosis in the context of predictive, preventive, and personalized medicine using integrative bioinformatics approaches and machine-learning strategies; Authors - Jinling Xu
human	IHC	Mol Oncol; Consensus molecular subtyping of colorectal carcinoma brain metastases reveals a metabolic signature associated with poor patient survival; Authors - Barnabas Irmer