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DHTKD1 Rekombinanter Antikörper

DHTKD1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251575H1

Kat-Nr. : 86661-3-RR

Synonyme

2-oxoadipate dehydrogenase complex component E1, 2-oxoadipate dehydrogenase, mitochondrial, Alpha-KADH-E1, Alpha-ketoadipate dehydrogenase, Dehydrogenase E1 and transketolase domain-containing protein 1

Filter:
  • Western Blot (WB) analysis of various lysates using DHTKD1 Recombinant monoclonal antibody (86661-3-RR)

    WB analysis using 86661-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86661-3-RR (DHTKD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86661-3-RR

    Affinity and Kinetic Characterization of 86661-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86661-3-RR against Human DHTKD1 were performed. The affinity constant is 1.99 nM.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBL02-Zellen, HepG2-Zellen, MCF-7-Zellen, Mausnierengewebe, Mauslebergewebe, Rattennierengewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

86661-3-RR bindet in WB, ELISA DHTKD1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen DHTKD1 fusion protein Ag26515
Vollständiger Name dehydrogenase E1 and transketolase domain containing 1
Berechnetes Molekulargewicht 103 kDa
Beobachtetes Molekulargewicht 103 kDa
GenBank-ZugangsnummerBC007955
Gene symbol DHTKD1
Gene ID (NCBI) 55526
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHTKD1 (Dehydrogenase E1 And Transketolase Domain Containing 1), also named as 2-aminoadipic 2-oxoadipic aciduria protein, OADC-E1, OADH-E1, is a lesser-studied E1 enzyme among the family of 2-oxoacid dehydrogenases (PMID: 32695416). DHTKD1 was expressed ubiquitously in various tissues and was proposed to localize to the mitochondria (PMID: 23141294). DHTKD1 plays a critical role in energy production in mitochondria, suppression of DHTKD1 leads to impaired mitochondrial biogenesis and increased cell apoptosis (PMID: 24076469).