DHX15 Polyklonaler Antikörper

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• KD/KO Validated

DHX15 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12265-1-AP

Synonyme

ATP dependent RNA helicase #46, DBP1, DDX15, DEAH box protein 15, DHX15, HRH2, PRP43, PRPF43, PrPp43p

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12265-1-AP

  WB result of DHX15 antibody (12265-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX15 transfected HeLa cells.

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  WB analysis using 12265-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12265-1-AP (DHX15 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12265-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12265-1-AP (DHX15 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12265-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12265-1-AP (DHX15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 12265-1-AP

  IP Result of anti-DHX15 (IP:12265-1-AP, 4ug; Detection:12265-1-AP 1:600) with NIH/3T3 cells lysate 2000ug.

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  IHC staining of mouse testis using 12265-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12265-1-AP (DHX15 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12265-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12265-1-AP (DHX15 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

12265-1-AP bindet in WB, IP, IHC, ELISA DHX15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHX15 fusion protein Ag2895
• Vollständiger Name: DEAH (Asp-Glu-Ala-His) box polypeptide 15
• Berechnetes Molekulargewicht: 795 aa, 91 kDa
• Beobachtetes Molekulargewicht: 91 kDa
• GenBank-Zugangsnummer: BC035974
• Gene symbol: DHX15
• Gene ID (NCBI): 1665
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHX15, also named as DBP1 and DDX15, is a DExD/H-box protein that has been identified in spliceosome, through its carboxyl (C)-terminal G-patch domain and stimulates the helicase activity of DHX15. DHX15 is a Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. The MW of DHX15 is about 90-95kd.

Publikationen

Species	Application	Title
zebrafish	WB	Cancer Sci; Dhx15 regulates zebrafish definitive hematopoiesis through the unfolded protein response pathway.; Authors - Yuanhua Cai
human	WB	J Cell Mol Med; ETS1 and SP1 drive DHX15 expression in acute lymphoblastic leukaemia.; Authors - Xiang-Lei Chen
human	WB	FEBS Lett; Tumor suppressor RBM5 directly interacts with the DExD/H-box protein DHX15 and stimulates its helicase activity.; Authors - Niu Zhaoyang Z
human	WB	RNA; A model for DHX15 mediated disassembly of A-complex spliceosomes; Authors - Hannah M Maul-Newby