DHX15 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

DHX15 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F13

Rezensionen (2)

Kat-Nr. : 82137-1-RR

Synonyme

EC:3.6.4.13, DDX15, DBP1, ATP-dependent RNA helicase DHX15, ATP-dependent RNA helicase #46

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  WB analysis of HeLa using 82137-1-RR

  WB result of DHX15 antibody (82137-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX15 transfected HeLa cells.

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  WB analysis using 82137-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82137-1-RR (DHX15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 82137-1-RR

  IP result of anti-DHX15 (IP:82137-1-RR, 4ug; Detection:82137-1-RR 1:8000) with NIH/3T3 cells lysate 1320 ug.

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  IHC staining of mouse testis using 82137-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82137-1-RR (DHX15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 82137-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82137-1-RR (DHX15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 82137-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 82137-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 82137-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:2500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 82137-1-RR

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DHX15 (82137-1-RR, Clone:1F13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 82137-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82137-1-RR against Human DHX15 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-251-Zellen, A431-Zellen, C6-Zellen, HeLa-Zellen, NIH/3T3-Zellen, U2OS-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82137-1-RR bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA DHX15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DHX15 fusion protein Ag2895
• Vollständiger Name: DEAH (Asp-Glu-Ala-His) box polypeptide 15
• Berechnetes Molekulargewicht: 795 aa, 91 kDa
• Beobachtetes Molekulargewicht: 90-95 kDa
• GenBank-Zugangsnummer: BC035974
• Gene symbol: DHX15
• Gene ID (NCBI): 1665
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHX15, also named as DBP1 and DDX15, is a DExD/H-box protein that has been identified in spliceosome, through its carboxyl (C)-terminal G-patch domain and stimulates the helicase activity of DHX15. DHX15 is a pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. The MW of DHX15 is about 90-95kDa.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (12-16-2024)

Great antibody for Dhx15 detection! On the picture, red asterisk indicates the non-specific band that comes from the previous round of staining with another antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: RKO

DHX15 Antibody Western Blot validation (1:10000 dilution) in RKO (Cat no:82137-1-RR)

FH

Tsimafei (Verified Customer) (08-12-2024)

Excellent and sensitive antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: mESCs

DHX15 Antibody Western Blot validation (1:10000 dilution) in mESCs (Cat no:82137-1-RR)