DHX32 Monoklonaler Antikörper

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• KD/KO Validated

DHX32 Monoklonal Antikörper für WB, ELISA, FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2A4G9

Kat-Nr. : 68306-1-Ig

Synonyme

HuDDX32, EC:3.6.4.13, DHLP1, DEAD/H helicase-like protein 1, DDX32

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  WB analysis using 68306-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 68306-1-Ig

  WB result of DHX32 antibody (68306-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX32 transfected A549 cells.

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  WB analysis using 68306-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 68306-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68306-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 68306-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug DHX32 Monoclonal antibody (68306-1-Ig, Clone:2A4G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen, Maushodengewebe, Rattenhodengewebe, U2OS-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

68306-1-Ig bindet in WB, FC (Intra), ELISA DHX32 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DHX32 fusion protein Ag13956
• Vollständiger Name: DEAH (Asp-Glu-Ala-His) box polypeptide 32
• Berechnetes Molekulargewicht: 743 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC002473
• Gene symbol: DHX32
• Gene ID (NCBI): 55760
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHX32, an RNA helicase, belongs to the DEAH box RNA helicase family by virtue of its homology to the conserved helicase domain. DHX32 is abundantly expressed in human and murine models, participating in cell transcription and differentiation, and being capable of regulating T-cell apoptosis. DHX32 is also associated with some malignant tumors, such as lymphoma, colorectal cancer, and breast cancer (PMID: 35117530). DHX32 has 2 isoforms with molecular weights of 75 and 84 kDa, respectively.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Combinatorial ubiquitin code degrades deubiquitylation-protected substrates; Authors - Mai Morita