DHX36 Polyklonaler Antikörper

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DHX36 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13159-1-PBS

Synonyme

G4R1, EC:3.6.4.12, DEAH-box protein 36, DEAD/H box polypeptide 36, DDX36

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  WB analysis using 13159-1-AP (same clone as 13159-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13159-1-AP (DHX36 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 13159-1-AP (same clone as 13159-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13159-1-AP (DHX36 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 13159-1-AP (same clone as 13159-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13159-1-AP (DHX36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 13159-1-AP (same clone as 13159-1-PBS)

  IP result of anti-DHX36 (IP:13159-1-AP, 4ug; Detection:13159-1-AP 1:500) with A431 cells lysate 1000 ug. This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 13159-1-AP (same clone as 13159-1-PBS)

  IP result of anti-DHX36 (IP:13159-1-AP, 4ug; Detection:13159-1-AP 1:500) with PC-3 cells lysate 1760 ug. This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13159-1-AP (same clone as 13159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13159-1-AP (same clone as 13159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13159-1-AP (same clone as 13159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 13159-1-AP (same clone as 13159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13159-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13159-1-PBS bindet in WB, IHC, IP, Indirect ELISA DHX36 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHX36 fusion protein Ag3799
• Vollständiger Name: DEAH (Asp-Glu-Ala-His) box polypeptide 36
• Berechnetes Molekulargewicht: 1008 aa, 115 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC036035
• Gene symbol: DHX36
• Gene ID (NCBI): 170506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHX36, also known as RNA associated with AU-rich element (RHAU) and G4R1, is an adenosine triphosphate (ATP)-dependent RNA helicase that belongs to the DExH/D family of RNA modifying enzymes. Proteins in this family involve in a wide range of cellular functions including RNA splicing, ribosome assembly, messenger RNA (mRNA) stability, microRNA processing, ribonucleoprotein remodeling and RNA trafficking. DHX36 is an RNA helicase that has specificity for DNA and RNA G4-quadruplexes, and resolves G4 structures into separate nucleotide strands for metabolic processing of G-rich sequence.