DHX38 Polyklonaler Antikörper

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• KD/KO Validated

DHX38 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10098-2-PBS

Synonyme

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  WB analysis using 10098-2-AP (same clone as 10098-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10098-2-AP (DHX38 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10098-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10098-2-AP (same clone as 10098-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10098-2-AP (DHX38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10098-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 10098-2-AP (same clone as 10098-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10098-2-AP (DHX38 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10098-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 10098-2-AP (same clone as 10098-2-PBS)

  IP result of anti-DHX38 (IP:10098-2-AP, 4ug; Detection:10098-2-AP 1:700) with HeLa cells lysate 1400ug. This data was developed using the same antibody clone with 10098-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10098-2-PBS bindet in WB, IP, Indirect ELISA DHX38 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHX38 fusion protein Ag0144
• Vollständiger Name: DEAH (Asp-Glu-Ala-His) box polypeptide 38
• Berechnetes Molekulargewicht: 141 kDa
• Beobachtetes Molekulargewicht: 141 kDa
• GenBank-Zugangsnummer: BC004235
• Gene symbol: DHX38
• Gene ID (NCBI): 9785
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The protein encoded by this gene is a member of the DEAD/H box family of splicing factors, which are involved in pre-mRNA splicing. The yeast prp16 is an essential splicing factor that catalyzes RNA-dependent hydrolysis of nucleoside triphosphates, acting as an ATPase. This protein resembles yeast Prp16 more closely than other DEAD/H family members and also is a general splicing factor essential for catalytic step II during pre-mRNA processing. Western blot analysis showed it is a 140-KD nuclear protein expressed in various tissues.

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