DHX58/LGP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DHX58/LGP2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 11355-1-AP

Synonyme

D11LGP2, D11lgp2e, DHX58, DHX58/LGP2, LGP2, Protein D11Lgp2 homolog, RIG I like receptor LGP2, RLR

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11355-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11355-1-AP (DHX58/LGP2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11355-1-AP

  IP Result of anti-DHX58/LGP2 (IP:11355-1-AP, 3ug; Detection:11355-1-AP 1:300) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of human breast cancer using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human nephroblastoma using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human nephroblastoma tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human nephroblastoma using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human nephroblastoma tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 11355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:10 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Rattenlebergewebe, Rattennierengewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nephroblastomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

11355-1-AP bindet in WB, IP, IHC, IF, ELISA DHX58/LGP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHX58/LGP2 fusion protein Ag1910
• Vollständiger Name: DEXH (Asp-Glu-X-His) box polypeptide 58
• Berechnetes Molekulargewicht: 678 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC014949
• Gene symbol: DHX58
• Gene ID (NCBI): 79132
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHX58(Probable ATP-dependent RNA helicase DHX58) is also named as D11LGP2E, LGP2 and belongs to the RLR subfamily. DHX58, originally identified as a highly expressed gene in mammary tumors, is another cytoplasmic DEX(D/H)-box helicase that can recognize RNA(PMID: 18411269). It acts as a positive, but not negative, regulator of RIG-I-and MDA5-dependent recognition of RNA virus infection and plays a pivotal role in antiviral responses in vivo(PMID:20080593).

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; RIG-I-like receptors direct inflammatory macrophage polarization against West Nile virus infection.; Authors - Amy E L Stone ; KO Validated
human	WB	Nucleic Acids Res; miR-122 does not impact recognition of the HCV genome by innate sensors of RNA but rather protects the 5' end from the cellular pyrophosphatases, DOM3Z and DUSP11.; Authors - Yalena Amador-Cañizares
human	WB	EMBO Rep; RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling.; Authors - Jean-Patrick Parisien
human,pig,monkey	WB,IF,IP	J Virol; LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling; Authors - Zhenbang Zhu ; KD Validated
human,mouse	WB	J Virol; Ambivalent role of the innate immune response in rabies virus pathogenesis.; Authors - Chopy Damien D
human,mouse	WB,IHC,IP	Cancer Sci; Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma.; Authors - Li-Ling Lin