DHX9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DHX9 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

35

Kat-Nr. : 17721-1-AP

Synonyme

ATP dependent RNA helicase A, DDX9, DEAH box protein 9, DHX9, LKP, NDH II, NDH2, NDHII, Nuclear DNA helicase II, RHA

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Galerie der Validierungsdaten

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  WB analysis using 17721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 17721-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 17721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17721-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 17721-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 17721-1-AP

  Immunofluorescent analysis of HepG2 cells, using DHX9 antibody 17721-1-AP at 1:100 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 17721-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Maushodengewebe, Rattenhodengewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 30 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below
• RIP: See 3 publications below

Produktinformation

17721-1-AP bindet in WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA DHX9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHX9 fusion protein Ag12104
• Vollständiger Name: DEAH (Asp-Glu-Ala-His) box polypeptide 9
• Berechnetes Molekulargewicht: 1270 aa, 141 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC014246
• Gene symbol: DHX9
• Gene ID (NCBI): 1660
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].

Publikationen

Species	Application	Title
human	IF	Hepatology; Splicing Regulator p54nrb /NONO Enhances Carcinogenesis Through Oncogenic Isoform Switch of BIN1 in Hepatocellular Carcinoma.; Authors - Zhixiang Hu
mouse	WB	Sci Adv; R-loops coordinate with SOX2 in regulating reprogramming to pluripotency.; Authors - Yaoyi Li
human	RIP	Nat Commun; Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1.; Authors - Hamed Kooshapur
human	WB	Nat Commun; Interaction of 7SK with the Smn complex modulates snRNP production.; Authors - Changhe Ji
human	WB	Nat Commun; TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.; Authors - Roy Matkovic
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu