DHX9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DHX9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(43)

Rezensionen (1)

Kat-Nr. : 17721-1-AP

Synonyme

NDH II, LKP, EC:3.6.4.13, DExH-box helicase 9, DDX9

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  WB analysis using 17721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 17721-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17721-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 17721-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 17721-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 17721-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 17721-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug DHX9 Polyclonal antibody (17721-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Maushodengewebe, Rattenmilzgewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 37 publications below
• IHC: See 4 publications below
• IF: See 8 publications below
• IP: See 3 publications below
• ELISA: See 1 publications below
• CoIP: See 4 publications below
• ChIP: See 1 publications below
• RIP: See 4 publications below

Produktinformation

17721-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA DHX9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHX9 fusion protein Ag12104
• Vollständiger Name: DEAH (Asp-Glu-Ala-His) box polypeptide 9
• Berechnetes Molekulargewicht: 1270 aa, 141 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC014246
• Gene symbol: DHX9
• Gene ID (NCBI): 1660
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].

Publikationen

Species	Application	Title
human	IF	Hepatology; Splicing Regulator p54nrb /NONO Enhances Carcinogenesis Through Oncogenic Isoform Switch of BIN1 in Hepatocellular Carcinoma.; Authors - Zhixiang Hu
mouse	WB	Sci Adv; R-loops coordinate with SOX2 in regulating reprogramming to pluripotency.; Authors - Yaoyi Li
human	RIP	Nat Commun; Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1.; Authors - Hamed Kooshapur
human	WB	Nat Commun; Interaction of 7SK with the Smn complex modulates snRNP production.; Authors - Changhe Ji
human	WB	Nat Commun; TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.; Authors - Roy Matkovic
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-12-2024)

Strong DHX9 band detected at the right size by WB (1/1000- 1h incubation at RT) with HeLa extracts.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa