DHX9 Monoklonaler Antikörper

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• KD/KO Validated

DHX9 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B12C10

Kat-Nr. : 67153-1-PBS

Synonyme

1B12C10, ATP-dependent RNA helicase A, DDX9, DExH-box helicase 9, EC:3.6.4.13

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  WB analysis using 67153-1-Ig (same clone as 67153-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67153-1-Ig (same clone as 67153-1-PBS)

  WB result of DHX9 antibody (67153-1-Ig; 1:400000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX9 transfected HEK-293 cells. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67153-1-Ig (same clone as 67153-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67153-1-Ig (same clone as 67153-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67153-1-Ig (same clone as 67153-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67153-1-Ig (same clone as 67153-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 67153-1-Ig (same clone as 67153-1-PBS)

  IP result of anti-DHX9 (IP:67153-1-Ig, 5ug; Detection:67153-1-Ig 1:20000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67153-1-Ig (same clone as 67153-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67153-1-Ig (same clone as 67153-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67153-1-Ig (same clone as 67153-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67153-1-Ig (same clone as 67153-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67153-1-Ig (same clone as 67153-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67153-1-Ig (DHX9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50161-1

  Cytometric bead array standard curve of MP50161-1, DHX9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67153-2-PBS. Detection antibody: 67153-1-PBS. Standard:Ag12104. Range: 1.563-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

67153-1-PBS bindet in WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA DHX9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DHX9 fusion protein Ag12104
• Vollständiger Name: DEAH (Asp-Glu-Ala-His) box polypeptide 9
• Berechnetes Molekulargewicht: 1270 aa, 141 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC014246
• Gene symbol: DHX9
• Gene ID (NCBI): 1660
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].