Smac/DIABLO Monoklonaler Antikörper

Smac/DIABLO Monoklonal Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F12C9

Kat-Nr. : 68480-1-PBS

Synonyme

DIABLO, 2F12C9, DFNA64, Diablo homolog, mitochondrial, Diablo IAP-binding mitochondrial protein

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  WB analysis using 68480-1-Ig (same clone as 68480-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68480-1-PBS in a different storage buffer formulation.

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  WB analysis using 68480-1-Ig (same clone as 68480-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68480-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68480-1-Ig (same clone as 68480-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Smac/DIABLO antibody (68480-1-Ig, Clone: 2F12C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68480-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50334-2

  Cytometric bead array standard curve of MP50334-2, DIABLO Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68480-4-PBS. Detection antibody: 68480-1-PBS. Standard:Ag32774. Range: 1.563-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68480-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA Smac/DIABLO und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Smac/DIABLO fusion protein Ag32774
• Vollständiger Name: diablo homolog (Drosophila)
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC011909
• Gene symbol: DIABLO
• Gene ID (NCBI): 56616
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Smac/DIABLO is a mitochondrial protein that promotes apoptosis by neutralizing members of the IAP family. SMAC/DIABLO is ubiquitously expressed in normal tissues, and the expression of SMAC messenger RNA (mRNA) in adult testis is the highest .Smac/DIABLO encodes a 239 amino acid protein (~27 kDa). The first 55 amino acids are a mitochondrial targeting signal peptide that is cleaved to generate mature Smac-DIABLO (~21 kDa). When released from mitochondria, Smac-DIABLO acts as a natural antagonist of inhibitor of apoptosis proteins (IAPs). Smac-DIABLO antagonistic activity is based on its N-terminal tetrapeptide (AVPI) that binds baculoviral IAP repeat (BIR) domains of IAPs, releasing their inhibitory effects on both initiator and effector caspases, thus promoting cell death. (PMID: 32325691, PMID: 32575872,PMID: 25650938)