DIAPH3 Polyklonaler Antikörper

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DIAPH3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14342-1-PBS

Synonyme

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  WB analysis of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

  WB result of DIAPH3 antibody (14342-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 14342-1-AP (same clone as 14342-1-PBS)

  WB result of DIAPH3 antibody (14342-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HEK-293 cells. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 14342-1-AP (same clone as 14342-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 14342-1-AP (same clone as 14342-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

  IP result of anti-DIAPH3 (IP:14342-1-AP, 4ug; Detection:14342-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 14342-1-AP (same clone as 14342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 14342-1-AP (same clone as 14342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 14342-1-AP (same clone as 14342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 14342-1-AP (same clone as 14342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 14342-1-AP (same clone as 14342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 14342-1-AP (same clone as 14342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DIAPH3 antibody (14342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

  Immunofluorescent analysis of HeLa cells using 14342-1-AP (DIAPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14342-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DIAPH3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DIAPH3 fusion protein Ag5656
• Vollständiger Name: diaphanous homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 137 kDa
• Beobachtetes Molekulargewicht: 125-137 kDa
• GenBank-Zugangsnummer: BC048963
• Gene symbol: DIAPH3
• Gene ID (NCBI): 81624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.