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DIAPH3 Rekombinanter Antikörper

DIAPH3 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242950G7

Kat-Nr. : 85701-1-PBS

Synonyme

DIAP3, Diaphanous-related formin-3, DRF3, MDia2

Filter:
  • Western Blot (WB) analysis of various lysates using DIAPH3 Recombinant antibody (85701-1-RR)

    WB analysis using 85701-1-RR (same clone as 85701-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85701-1-RR (DIAPH3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85701-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DIAPH3 Recombinant antibody (85701-1-RR)

    WB analysis of HeLa using 85701-1-RR (same clone as 85701-1-PBS)

    WB result of DIAPH3 antibody (85701-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells. This data was developed using the same antibody clone with 85701-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DIAPH3 Recombinant antibody (85701-1-RR)

    IF Staining of U2OS using 85701-1-RR (same clone as 85701-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using DIAPH3 antibody (85701-1-RR, Clone: 242950G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85701-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85701-1-RR

    Affinity and Kinetic Characterization of 85701-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85701-1-RR against Human DIAPH3 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

85701-1-PBS bindet in WB, IF/ICC, Indirect ELISA DIAPH3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen DIAPH3 fusion protein Ag6631
Vollständiger Name diaphanous homolog 3 (Drosophila)
Berechnetes Molekulargewicht 137 kDa
Beobachtetes Molekulargewicht 140 kDa
GenBank-ZugangsnummerBC048963
Gene symbol DIAPH3
Gene ID (NCBI) 81624
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.