DIAPH3 Rekombinanter Antikörper

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• KD/KO Validated

DIAPH3 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242950G7

Kat-Nr. : 85701-1-RR

Synonyme

DIAP3, Diaphanous-related formin-3, DRF3, MDia2

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  WB analysis using 85701-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85701-1-RR (DIAPH3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 85701-1-RR

  WB result of DIAPH3 antibody (85701-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells.

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  IF Staining of U2OS using 85701-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using DIAPH3 antibody (85701-1-RR, Clone: 242950G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85701-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85701-1-RR against Human DIAPH3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, DU 145-Zellen, HEK-293-Zellen, MCF-7-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85701-1-RR bindet in WB, IF/ICC, ELISA DIAPH3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DIAPH3 fusion protein Ag6631
• Vollständiger Name: diaphanous homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 137 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC048963
• Gene symbol: DIAPH3
• Gene ID (NCBI): 81624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.