DICER1 Monoklonaler Antikörper

DICER1 Monoklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C8E2

Kat-Nr. : 68375-1-PBS

Synonyme

DCR1, Dicer, dicer 1, ribonuclease type III, DICER1, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase motif, HERNA, KIAA0928

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  WB analysis of U2OS using 68375-1-Ig (same clone as 68375-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68375-1-Ig (DICER1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68375-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 68375-1-Ig (same clone as 68375-1-PBS)

  IP result of anti-DICER1 (IP:68375-1-Ig, 4ug; Detection:68375-1-Ig 1:500) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 68375-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68375-1-Ig (same clone as 68375-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DICER1 antibody (68375-1-Ig, Clone: 1C8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68375-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68375-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA DICER1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DICER1 fusion protein Ag30368
• Vollständiger Name: dicer 1, ribonuclease type III
• Berechnetes Molekulargewicht: 219 kDa
• Beobachtetes Molekulargewicht: 240-250 kDa
• GenBank-Zugangsnummer: NM_030621
• Gene symbol: DICER1
• Gene ID (NCBI): 23405
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DICER1 functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. DICER1 cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, and producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation.