DIDO1 Polyklonaler Antikörper

DIDO1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10183-1-PBS

Synonyme

BYE1, C20orf158, DATF 1, DATF1, DATF-1

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  WB analysis of Raji using 10183-1-AP (same clone as 10183-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10183-1-AP (DIDO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10183-1-AP (same clone as 10183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10183-1-AP (DIDO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10183-1-AP (same clone as 10183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10183-1-AP (DIDO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10183-1-AP (same clone as 10183-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DIDO1 antibody (10183-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10183-1-AP (same clone as 10183-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using DIDO1 antibody 10183-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10183-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DIDO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DIDO1 fusion protein Ag0237
• Vollständiger Name: death inducer-obliterator 1
• Berechnetes Molekulargewicht: 244 kDa
• Beobachtetes Molekulargewicht: 300-350 kDa
• GenBank-Zugangsnummer: BC000770
• Gene symbol: DIDO1
• Gene ID (NCBI): 11083
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DIDO1, also termed DATF-1, C20orf158, DATF1, KIAA0333 and DIO-1, is a putative transcription factor, weakly pro-apoptotic when overexpressed. It is a tumor suppressor. In mice, DIDO1 is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. The human DIDO1 is similar to the mouse gene and therefore is thought to be involved in apoptosis. DIDO1 has multiple isoforms with molecular weights of 243 kd, 129 kd, 59 kd, and 61 kd. DIDO1 undergoes various modifications, including phosphorylation and ubiquitination, resulting in a mainstream molecular weight of 300 kd.

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