DIO1 Polyklonaler Antikörper

DIO1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 11790-1-AP

Synonyme

5DI, DIOI, EC:1.21.99.4, ITDI1, TXDI1

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  WB analysis of mouse lung using 11790-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11790-1-AP (DIO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 11790-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11790-1-AP (DIO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11790-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11790-1-AP (DIO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 11790-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 11790-1-AP (DIO1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11790-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11790-1-AP (DIO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11790-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11790-1-AP (DIO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 11790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11790-1-AP (DIO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11790-1-AP (DIO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11790-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11790-1-AP (DIO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11790-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11790-1-AP (DIO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11790-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11790-1-AP (DIO1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11790-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11790-1-AP (DIO1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 11790-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using DIO1 antibody (11790-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11790-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DIO1 Polyclonal antibody (11790-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, A549-Zellen, HeLa-Zellen, Maus-Eierstockgewebe, Rattenlungengewebe, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

11790-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA DIO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DIO1 fusion protein Ag2410
• Vollständiger Name: deiodinase, iodothyronine, type I
• Berechnetes Molekulargewicht: 249 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa, 13 kDa
• GenBank-Zugangsnummer: BC017955
• Gene symbol: DIO1
• Gene ID (NCBI): 1733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DIO1(Type I iodothyronine deiodinase) is also named as ITDI1, TXDI1,5DI,DIOI and belongs to the iodothyronine deiodinase family.It is a membrane selenoprotein that catalyzes the deiodination of L-thyroxine to the biologically active thyroid hormone 3,3′,5-triiodothyronine.DIO1 is located in liver, kidney, and thyroid,which has both ORD and IRD activities(PMID: 9389495). It has some isoforms produced by alternative splicing with the MW of 29 kDa, 21 kDa, 13 kDa, 23 kDa, 19 kDa, 4 kDa, 13 kDa, 9 kDa and 10 kDa.

Publikationen

Species	Application	Title
mouse	IF	Cell Rep; Environmental Enrichment Induces Pericyte and IgA-Dependent Wound Repair and Lifespan Extension in a Colon Tumor Model.; Authors - Benjamin D Bice
rat	WB,IHC	Chemosphere; Rno-miR-224-5p contributes to 2,2',4,4'-tetrabromodiphenyl ether-induced low triiodothyronine in rats by targeting deiodinases.; Authors - Chao Wang
mouse,human	WB,IHC	J Biol Chem; Astrocyte Elevated Gene-1 (AEG-1) Contributes to Nonthyroidal Illness Syndrome (NTIS) Associated with Hepatocellular Carcinoma (HCC).; Authors - Jyoti Srivastava
mouse	IF	Sci Rep; Sex-specific transgenerational effects on murine thyroid gland imposed by ancestral exposure to neonicotinoid thiacloprid; Authors - Mariam Diba Lahmidi
mouse	WB	Sci Rep; Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets.; Authors - B Chellan
rat	WB	Front Physiol; Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding.; Authors - Paula B M De Andrade