DIO2 Polyklonaler Antikörper

DIO2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 26513-1-AP

Synonyme

5DII, D2, DIOII, EC:1.21.99.4, ITDI2

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  WB analysis of mouse skeletal muscle using 26513-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 26513-1-AP (DIO2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26513-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26513-1-AP (DIO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 26513-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26513-1-AP (DIO2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 26513-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26513-1-AP (DIO2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 26513-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIO2 antibody (26513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IHC: See 2 publications below

Produktinformation

26513-1-AP bindet in WB, IHC, IF/ICC, ELISA DIO2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DIO2 fusion protein Ag24119
• Vollständiger Name: deiodinase, iodothyronine, type II
• Berechnetes Molekulargewicht: 273 aa, 31 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC074882
• Gene symbol: DIO2
• Gene ID (NCBI): 1734
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type II iodothyronine deiodinase(DIO2), belongs to the iodothyronine deiodinase family, with two isoform of 30 and 34 kDa. DIO2 can activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). DIO2 is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues.

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); Tumor Cell "Slimming" Regulates Tumor Progression through PLCL1/UCP1-Mediated Lipid Browning.; Authors - Zhiyong Xiong
human	WB	Nat Chem Biol; Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability.; Authors - Zhipeng Li
mouse	WB	Diabetes; Id1 Promotes Obesity by Suppressing Brown Adipose Thermogenesis and White Adipose Browning.; Authors - Mallikarjun Patil
human	WB,IHC	Clin Transl Med; NNT-induced tumor cell "slimming" reverses the pro-carcinogenesis effect of HIF2a in tumors.; Authors - Zhiyong Xiong
mouse	WB	Ecotoxicol Environ Saf; Triiodothyronine activates THRβ to promote PGC1α expression alleviating PQ-induced pulmonary fibrosis; Authors - Wenbo Chen
mouse	WB	FASEB J; The tumor secretory factor ZAG promotes white adipose tissue browning and energy wasting.; Authors - Sawsan Elattar