DIO2 Monoklonaler Antikörper

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• KD/KO Validated

DIO2 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F2A1

Kat-Nr. : 66813-1-PBS

Synonyme

5DII, D2, DIOII, EC:1.21.99.4, ITDI2

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• 

  WB analysis of T-47D using 66813-1-Ig (same clone as 66813-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66813-1-Ig (DIO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66813-1-Ig (same clone as 66813-1-PBS)

  WB result of DIO2 antibody (66813-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIO2 transfected MCF-7 cells. This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 66813-1-Ig (same clone as 66813-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66813-1-Ig (DIO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 66813-1-Ig (same clone as 66813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 66813-1-Ig (same clone as 66813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66813-1-PBS bindet in WB, IHC, ELISA DIO2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DIO2 fusion protein Ag24057
• Vollständiger Name: deiodinase, iodothyronine, type II
• Berechnetes Molekulargewicht: 273 aa, 31 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC074882
• Gene symbol: DIO2
• Gene ID (NCBI): 1734
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type II iodothyronine deiodinase(DIO2), belongs to the iodothyronine deiodinase family, with two isoform of 30 and 34 kDa. DIO2 can activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). DIO2 is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues.