DIS3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DIS3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Rezensionen (1)

Kat-Nr. : 14689-1-AP

Synonyme

KIAA1008, EXOSC11, EC:3.1.13.-, dis3p, Dis-3

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  WB analysis using 14689-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14689-1-AP (DIS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 14689-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14689-1-AP (DIS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14689-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14689-1-AP (DIS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 14689-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14689-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14689-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIS3 antibody (14689-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 14689-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14689-1-AP (DIS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, Maus-Eierstockgewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14689-1-AP bindet in WB, IHC, IF/ICC, ELISA DIS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, drosophila, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DIS3 fusion protein Ag6117
• Vollständiger Name: DIS3 mitotic control homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 109 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC056143
• Gene symbol: DIS3
• Gene ID (NCBI): 22894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DIS3, also named as KIAA1008 and RRP44, belongs to the ribonuclease II (RNB) family. It is a component of the exosome 3'->5' exoribonuclease complex. DIS3 is required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. It is also associated with the GTPase Ran. DIS3 has a 3'-5' exonuclease activity.

Publikationen

Species	Application	Title
mouse	WB	Nature; A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.; Authors - Chang Hao-Ming HM
human	WB	Mol Cell; DGCR8 Acts as an Adaptor for the Exosome Complex to Degrade Double-Stranded Structured RNAs.; Authors - Sara Macias
human	WB	Sci Adv; Enhanced packaging of U6 small nuclear RNA and splicing-related proteins into extracellular vesicles during HIV infection; Authors - Yiyao Huang
mouse	WB	Nucleic Acids Res; RNA-regulatory exosome complex confers cellular survival to promote erythropoiesis.; Authors - Charu Mehta
mouse	IF	Proc Natl Acad Sci U S A; The early macrophage response to pathogens requires dynamic regulation of the nuclear paraspeckle; Authors - Sikandar Azam ; KD Validated
human	WB	EMBO J; The RNA exosome complex degrades expanded hexanucleotide repeat RNA in C9orf72 FTLD/ALS.; Authors - Yuya Kawabe ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (07-15-2022)

30µg of total protein lysate were loaded on the gel. Primary antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

DIS3 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:14689-1-AP)