DIS3 Polyklonaler Antikörper

DIS3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29664-1-PBS

Synonyme

DIS 3, Dis-3, dis3p, EC:3.1.13.-, EXOSC11

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  WB analysis using 29664-1-AP (same clone as 29664-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29664-1-AP (DIS3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 29664-1-AP (same clone as 29664-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIS3 antibody (29664-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29664-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DIS3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DIS3 fusion protein Ag31711
• Vollständiger Name: DIS3 mitotic control homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 109KD
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: NM_014953
• Gene symbol: DIS3
• Gene ID (NCBI): 22894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DIS3, also named as KIAA1008 and RRP44, belongs to the ribonuclease II (RNB) family. It is a component of the exosome 3'->5' exoribonuclease complex. DIS3 is required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. It is also associated with the GTPase Ran. DIS3 has a 3'-5' exonuclease activity.