DKK1 Polyklonaler Antikörper

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• KD/KO Validated

DKK1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

31

Kat-Nr. : 21112-1-AP

Synonyme

Dickkopf 1, Dickkopf related protein 1, DKK 1, DKK1, hDkk 1, SK

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Galerie der Validierungsdaten

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  WB analysis using 21112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21112-1-AP (DKK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21112-1-AP (DKK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 21112-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 21112-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 21112-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 21112-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 21112-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 6 publications below

Produktinformation

21112-1-AP bindet in WB, IHC, ELISA DKK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DKK1 fusion protein Ag15110
• Vollständiger Name: dickkopf homolog 1 (Xenopus laevis)
• Berechnetes Molekulargewicht: 266 aa, 29 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC001539
• Gene symbol: DKK1
• Gene ID (NCBI): 22943
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kDa doublet protein, which is larger than the deduced molecular mass of 26 kDa. Sometime DKK1 is expressed as a 42- to 50-kDa secreted protein, with little change observed after glycanase treatment.

Publikationen

Species	Application	Title
human,mouse	WB	Nat Commun; Cancer stem cell regulated phenotypic plasticity protects metastasized cancer cells from ferroptosis.; Authors - Mingming Wu ; KD Validated
human	WB	Bioengineered; Exosomes from bone marrow mesenchymal stem cells promoted osteogenic differentiation by delivering miR-196a that targeted Dickkopf-1 to activate Wnt/β-catenin pathway; Authors - Zhi Peng
human	WB	Oncotarget; MicroRNA-372 inhibits endometrial carcinoma development by targeting the expression of the Ras homolog gene family member C (RhoC).; Authors - Bo-Liang Liu
rat	WB	Cells; Sirtuin 6 Overexpression Improves Rotator Cuff Tendon-to-Bone Healing in the Aged; Authors - Young Jae Moon
rat	WB	Osteoporos Int; Rehmanniae Radix Preparata suppresses bone loss and increases bone strength through interfering with canonical Wnt/β-catenin signaling pathway in OVX rats.; Authors - C Liu
human	WB	Biochem Pharmacol; miR-29a-3p-dependent COL3A1 and COL5A1 expression reduction assists sulforaphane to inhibit gastric cancer progression.; Authors - Sichong Han

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