DKK1 Rekombinanter Antikörper

DKK1 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250658B2

Kat-Nr. : 86207-1-PBS

Synonyme

Dickkopf-1, Dickkopf-related protein 1, DKK 1, Dkk-1, hDkk 1

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  WB analysis using 86207-1-RR (same clone as 86207-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86207-1-RR (DKK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86207-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02491-1

  Sandwich ELISA standard curve of MP02491-1, Mouse DKK1 Recombinant Matched Antibody Pair - PBS only. 86207-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4649. 86207-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP02491-1

  Serum of eight mice was measured. The mouse DKK1 concentration of detected samples was determined to be 4.72 ng/mL with a range of 2.40 - 7.06 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86207-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test DKK1 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DKK1 fusion protein Eg4649
• Vollständiger Name: dickkopf homolog 1 (Xenopus laevis)
• Berechnetes Molekulargewicht: 29kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: NM_010051.3
• Gene symbol: Dkk1
• Gene ID (NCBI): 13380
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kDa doublet protein, which is larger than the deduced molecular mass of 26 kDa. Sometime DKK1 is expressed as a 42- to 50-kDa secreted protein, with little change observed after glycanase treatment.