DLAT Polyklonaler Antikörper

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DLAT Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13426-1-PBS

Synonyme

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLTA, EC:2.3.1.12, ODP2, PDC-E2

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  WB analysis using 13426-1-AP (same clone as 13426-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13426-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13426-1-AP (same clone as 13426-1-PBS)

  WB result of DLAT antibody (13426-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLAT transfected HeLa cells. This data was developed using the same antibody clone with 13426-1-PBS in a different storage buffer formulation.

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  WB analysis using 13426-1-AP (same clone as 13426-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13426-1-PBS in a different storage buffer formulation.

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  WB analysis of HAP1 using 13426-1-AP (same clone as 13426-1-PBS)

  WB result of DLAT antibody (13426-1-AP; 1:60000; room temperature for 1.5 hours) with wild-type and DLAT knockout HAP1 cells. This data was developed using the same antibody clone with 13426-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 13426-1-AP (same clone as 13426-1-PBS)

  IP result of anti-DLAT (IP:13426-1-AP, 3ug; Detection:13426-1-AP 1:1000) with HepG2 cells lysate 2000ug. This data was developed using the same antibody clone with 13426-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13426-1-AP (same clone as 13426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13426-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13426-1-AP (same clone as 13426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13426-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 13426-1-AP (same clone as 13426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13426-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 13426-1-AP (same clone as 13426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13426-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 13426-1-AP (same clone as 13426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13426-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13426-1-AP (same clone as 13426-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DLAT antibody (13426-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13426-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13426-1-AP (same clone as 13426-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (13426-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 13426-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13426-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DLAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DLAT fusion protein Ag4226
• Vollständiger Name: dihydrolipoamide S-acetyltransferase
• Berechnetes Molekulargewicht: 647 aa, 70 kDa
• Beobachtetes Molekulargewicht: 69-70 kDa
• GenBank-Zugangsnummer: BC039084
• Gene symbol: DLAT
• Gene ID (NCBI): 1737
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.