DLAT Rekombinanter Antikörper

DLAT Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240713C12

Kat-Nr. : 83654-3-RR

Synonyme

PDC-E2, ODP2, EC:2.3.1.12, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

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  WB analysis using 83654-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83654-3-RR (DLAT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 83654-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83654-3-RR (DLAT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IF Staining of HepG2 using 83654-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (83654-3-RR, Clone: 240713C12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 83654-3-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug DLAT Recombinant Antibody (83654-3-RR, Clone:240713C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 83654-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DLAT Recombinant antibody (83654-3-RR, Clone:240713C12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), or 0.25 ug DLAT Recombinant antibody (83654-3-RR, Clone: 240713C12) (green). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83654-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83654-3-RR against Human DLAT were performed. The affinity constant is 0.17 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen, MCF-7-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83654-3-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA DLAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DLAT fusion protein Ag22831
• Vollständiger Name: dihydrolipoamide S-acetyltransferase
• Berechnetes Molekulargewicht: 647 aa, 70 kDa
• Beobachtetes Molekulargewicht: 69-70 kDa
• GenBank-Zugangsnummer: BC039084
• Gene symbol: DLAT
• Gene ID (NCBI): 1737
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.