DLC1 Monoklonaler Antikörper

DLC1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G10H12

Kat-Nr. : 66894-1-PBS

Synonyme

ARHGAP7, deleted in liver cancer 1, DLC 1, DLC1, FLJ21120, HP, HP protein, KIAA1723, p122 RhoGAP, STARD12

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  WB analysis using 66894-1-Ig (same clone as 66894-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66894-1-Ig (DLC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66894-1-Ig (DLC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

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  IF Staining of A375 using 66894-1-Ig (same clone as 66894-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using DLC1 antibody (66894-1-Ig, Clone: 1G10H12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DLC1 antibody (66894-1-Ig, Clone: 1G10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DLC1 antibody (66894-1-Ig, Clone: 1G10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66894-1-PBS bindet in WB, IF/ICC, Indirect ELISA DLC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DLC1 fusion protein Ag24404
• Vollständiger Name: deleted in liver cancer 1
• Berechnetes Molekulargewicht: 171 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC054511
• Gene symbol: DLC1
• Gene ID (NCBI): 10395
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Deleted in Liver Cancer 1 (DLC1) is a RhoGAP-containing tumor suppressor that inhibits angiogenesis by repressing VEGF production in epithelial cells. DLC1 is often down-regulated in a variety of cancers, such as those of the liver, lung, breast, stomach, brain, colon and prostate due to either genomic deletion or aberrant DNA methylation.