DLD Monoklonaler Antikörper

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DLD Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E7G5

Kat-Nr. : 67702-1-PBS

Synonyme

2E7G5, DLDH, EC:1.8.1.4, GCSL, LAD

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  WB analysis using 67702-1-Ig (same clone as 67702-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67702-1-Ig (same clone as 67702-1-PBS)

  WB result of DLD antibody (67702-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLD transfected HeLa cells. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67702-1-Ig (same clone as 67702-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67702-1-Ig (same clone as 67702-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67702-1-PBS bindet in WB, IHC, Indirect ELISA DLD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DLD fusion protein Ag9680
• Vollständiger Name: dihydrolipoamide dehydrogenase
• Berechnetes Molekulargewicht: 509 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC018696
• Gene symbol: DLD
• Gene ID (NCBI): 1738
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.