DLG5 Polyklonaler Antikörper

DLG5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 15687-1-AP

Synonyme

Placenta and prostate DLG, PDLG, KIAA0583, Disks large homolog 5, Discs large protein P-dlg

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  WB analysis of PC-3 using 15687-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15687-1-AP (DLG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15687-1-AP (DLG5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15687-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15687-1-AP (DLG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of Human bowens disease using 15687-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 15687-1-AP (DLG5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Human bowens disease using 15687-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 15687-1-AP (DLG5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HeLa-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: Human bowens disease; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

15687-1-AP bindet in WB, IHC, ELISA DLG5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DLG5 fusion protein Ag8194
• Vollständiger Name: discs, large homolog 5 (Drosophila)
• Berechnetes Molekulargewicht: 1893 aa, 209 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 240 kDa
• GenBank-Zugangsnummer: BC002915
• Gene symbol: DLG5
• Gene ID (NCBI): 9231
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Discs large homolog 5 (DLG5) belongs to the membrane-associated guanylate kinase (MAGUK) family. DLG5 participates in epithelial cell polarity maintenance and cancer development. DLG5 is highly expressed in normal tissues, but its expression is decreased in many cancers, such as bladder cancer, prostate cancer, breast cancer, and hepatocellular carcinoma (PMID: 32015336). Down-regulation of DLG5 is highly correlated with clinical tumor stage. In breast cancer, knockdown of DLG5 induces cell migration, and overexpression of DLG5 inhibits cell migration (PMID: 28169360). DLG5 has 3 isoforms with the molecular weight of 75, 214 and 240 kDa.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay.; Authors - Rui Su

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rebecca (Verified Customer) (10-25-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC. I obtained several bands: around 105 kDa, 200 kDa and 240 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: iPSC-CM

DLG5 Antibody Western Blot validation (1:1000 dilution) in iPSC-CM (Cat no:15687-1-AP)