HURP Polyklonaler Antikörper

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• KD/KO Validated

HURP Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12038-1-AP

Synonyme

DAP 5, Discs large homolog 7, DLG7, DLGAP5, Hepatoma up regulated protein, HURP, KIAA0008

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 12038-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12038-1-AP (DLGAP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12038-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12038-1-AP (DLGAP5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 12038-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 12038-1-AP (HURP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

12038-1-AP bindet in WB, IHC, IF, ELISA HURP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HURP fusion protein Ag2667
• Vollständiger Name: discs, large (Drosophila) homolog-associated protein 5
• Berechnetes Molekulargewicht: 846 aa, 95 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC010658
• Gene symbol: DLGAP5
• Gene ID (NCBI): 9787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DLGAP5, also named as DAP 5 or DLG7, is a kinetochore protein that stabilizes microtubules in vicinity of chromosomes. DLG7 controls spindle dynamics, promotes interkinetochore tension and efficient kinetochore capture. DGL7 is a part of Ran-dependent complex. Expression of DGL7 is found in cancer and stem cells. DLGAP5 contributes to hepatocellular carcinoma tumorigenesis by promoting cell proliferation (PMID: 24324629).

Publikationen

Species	Application	Title
human	IHC	J Transl Med; Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance; Authors - Kun Zheng
pig	IF	Cells; Mitotic Acetylation of Microtubules Promotes Centrosomal PLK1 Recruitment and Is Required to Maintain Bipolar Spindle Homeostasis.; Authors - Sylvia Fenosoa Rasamizafy
human	WB,IHC	Life Sci; Pan-cancer analysis and experiments with cell lines reveal that the slightly elevated expression of DLGAP5 is involved in clear cell renal cell carcinoma progression.; Authors - Yun Feng ; KD Validated
human	WB	Front Endocrinol (Lausanne); Whole Genome Transcriptomic Analysis of Ovary Granulosa Cells Revealed an Anti-Apoptosis Regulatory Gene DLGAP5 in Polycystic Ovary Syndrome.; Authors - Yan Deng
human	WB,IHC	Dis Markers; High Expression of DLGAP5 Indicates Poor Prognosis and Immunotherapy in Lung Adenocarcinoma and Promotes Proliferation through Regulation of the Cell Cycle; Authors - Xiaolong Tang ; KD Validated