DLK1 Monoklonaler Antikörper

DLK1 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2C3H8

Kat-Nr. : 67923-1-Ig

Synonyme

DLK, DLK 1, DLK1, FA1, pG2, Pref 1, PREF1, Protein delta homolog 1, ZOG

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Galerie der Validierungsdaten

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  WB analysis using 67923-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67923-1-Ig (DLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67923-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67923-1-Ig (DLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig adrenal gland using 67923-1-Ig

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67923-1-Ig (DLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67923-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67923-1-Ig (DLK1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67923-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67923-1-Ig (DLK1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: JAR-Zellen, Hausschwein-Nebennierengewebe, humanes Plazenta-Gewebe, NCCIT-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67923-1-Ig bindet in WB, IHC, ELISA DLK1 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DLK1 fusion protein Ag30916
• Vollständiger Name: delta-like 1 homolog (Drosophila)
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 45-60 kDa
• GenBank-Zugangsnummer: BC007741
• Gene symbol: DLK1
• Gene ID (NCBI): 8788
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DLK1, also named PREF1, FA1, or pG2, is a transmembrane protein belonging to the epidermal growth factor (EGF)-like superfamily (PMID: 8095043). It contains six EGF-like repeats in the extracellular region. DLK1 is abundant in preadipocytes and regulate adipocyte differentiation negatively (PMID: 8500166). Deficiency of DLK1 gives rise to growth retardation and accelerated adiposity in mouse model. Expression of DLK1 is found in tumors with neuroendocrine features that implies DLK1 may be involved in neuroendocrine differentiation (PMID: 8095043). It has been reported overexpression of DLK1 could lead to the development of metabolic abnormalities by impairment of adipocyte function in mice (PMID: 12588883). The gene of DLK1 maps to chromosome 14q32, and encodes a 383-amino acid protein with a calculated molecular mass of 41 kDa. In preadipocytes, multiple discrete forms of DLK1 protein of 45-60 kDa are present, owing in part to N-linked glycosylation (PMID: 8500166).