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DLK2 Polyklonaler Antikörper

DLK2 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15680-1-AP

Synonyme

DLK 2, DLK2, EGF like protein 9, EGFL9, Protein delta homolog 2

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using DLK2 Polyclonal antibody (15680-1-AP)

    WB analysis of mouse brain using 15680-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15680-1-AP (DLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using DLK2 Polyclonal antibody (15680-1-AP)

    WB analysis of human liver using 15680-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15680-1-AP (DLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DLK2 Polyclonal antibody (15680-1-AP)

    IP experiment of mouse brain using 15680-1-AP

    IP result of anti-DLK2 (IP:15680-1-AP, 4ug; Detection:15680-1-AP 1:500) with mouse brain tissue lysate 5600 ug.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, humanes Lebergewebe
Erfolgreiche IPMaushirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 2 publications below

Produktinformation

15680-1-AP bindet in WB, IP, ELISA DLK2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DLK2 fusion protein Ag8170
Vollständiger Name delta-like 2 homolog (Drosophila)
Berechnetes Molekulargewicht383aa,41 kDa; 204aa,21 kDa
Beobachtetes Molekulargewicht 41 kDa
GenBank-ZugangsnummerBC006425
Gene symbol DLK2
Gene ID (NCBI) 65989
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
WB protocol for DLK2 antibody 15680-1-APProtokoll herunterladen
IP protocol for DLK2 antibody 15680-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Different Expression Levels of DLK2 Inhibit NOTCH Signaling and Inversely Modulate MDA-MB-231 Breast Cancer Tumor Growth In Vivo.

Authors - Ana-Isabel Naranjo
View Article
mouse

Sci Rep

DLK proteins modulate NOTCH signaling to influence a brown or white 3T3-L1 adipocyte fate.

Authors - María-Luisa Nueda
View Article
ratWB

Tissue Cell

Delta like Non-Canonical Notch Ligand 2 inhibits chondrogenic differentiation and cell proliferation of bone marrow mesenchymal stem cells through the Notch1 signaling pathway

Authors - Yanan Chen
View Article