DLK2 Polyklonaler Antikörper

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• KD/KO Validated

DLK2 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15680-1-AP

Synonyme

Epidermal growth factor-like protein 9, EGF-like protein 9, EGFL9, EGF like protein 9, DLK-2

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  WB analysis of mouse brain using 15680-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15680-1-AP (DLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15680-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15680-1-AP (DLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15680-1-AP

  IP result of anti-DLK2 (IP:15680-1-AP, 4ug; Detection:15680-1-AP 1:500) with mouse brain tissue lysate 5600 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Lebergewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

15680-1-AP bindet in WB, IP, ELISA DLK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DLK2 fusion protein Ag8170
• Vollständiger Name: delta-like 2 homolog (Drosophila)
• Berechnetes Molekulargewicht: 383aa,41 kDa; 204aa,21 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC006425
• Gene symbol: DLK2
• Gene ID (NCBI): 65989
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Different Expression Levels of DLK2 Inhibit NOTCH Signaling and Inversely Modulate MDA-MB-231 Breast Cancer Tumor Growth In Vivo.; Authors - Ana-Isabel Naranjo
sheep	WB	J Cell Physiol; Delta-like noncanonical notch ligand 2 regulates the proliferation and differentiation of sheep myoblasts through the Wnt/β-catenin signaling pathway; Authors - Lixia Sun ; KD Validated
mouse		Sci Rep; DLK proteins modulate NOTCH signaling to influence a brown or white 3T3-L1 adipocyte fate.; Authors - María-Luisa Nueda
rat	WB	Tissue Cell; Delta like Non-Canonical Notch Ligand 2 inhibits chondrogenic differentiation and cell proliferation of bone marrow mesenchymal stem cells through the Notch1 signaling pathway; Authors - Yanan Chen