DLL4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DLL4 Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21584-1-PBS

Synonyme

Delta4, hdelta2, UNQ1895/PRO4341

---

• 

  WB analysis using 21584-1-AP (same clone as 21584-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 21584-1-AP (same clone as 21584-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

  IP result of anti-DLL4 (IP:21584-1-AP, 4ug; Detection:21584-1-AP 1:300) with mouse lung tissue lysate 4000 ug. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human stomach cancer using 21584-1-AP (same clone as 21584-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using 21584-1-AP (DLL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

21584-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA DLL4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DLL4 fusion protein Ag16140
• Vollständiger Name: delta-like 4 (Drosophila)
• Berechnetes Molekulargewicht: 685 aa, 75 kDa
• Beobachtetes Molekulargewicht: 70-100 kDa
• GenBank-Zugangsnummer: BC106950
• Gene symbol: DLL4
• Gene ID (NCBI): 54567
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DLL4, also named as Delta4, plays a role in the Notch signaling pathway. It activates Notch-1 and Notch-4. Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. DLL4 is highly expressed in the vascular endothelium and is involved in angiogenesis.