DLX5 Monoklonaler Antikörper

DLX5 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C9G1

Kat-Nr. : 67111-1-Ig

Synonyme

distal less homeobox 5, DLX5, Homeobox protein DLX 5

---

Galerie der Validierungsdaten

• 

  WB analysis using 67111-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 67111-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 67111-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67111-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67111-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67111-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67111-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67111-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, A375-Zellen, Hausschwein-Hirngewebe, Maushirngewebe, Rattenhirngewebe, THP-1-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67111-1-Ig bindet in WB, IHC, ELISA DLX5 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DLX5 fusion protein Ag17040
• Vollständiger Name: distal-less homeobox 5
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 33-38 kDa
• GenBank-Zugangsnummer: BC006226
• Gene symbol: DLX5
• Gene ID (NCBI): 1749
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DLX5 belongs to the DLX family of homeobox transcription factors (Dlx1, Dlx2, Dlx5 and Dlx6), which is expressed in overlapping domains at different stages of cell differentiation in the subpallium and controls differentiation of GABAergic neurons. DLX5 is an immediate early BMP-responsive transcriptional activator that essential for osteoblast differentiation. It can bind to and stimulate the homeodomain-response element of the ALPL and SP7 promoter. DLX5 can form homodimer with DLX2(~34kd) and heterodimer with MSX2(~29kd).