DMC1 Monoklonaler Antikörper

DMC1 Monoklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D5C9

Kat-Nr. : 67176-1-PBS

Synonyme

LIM15, HsLim15, DMC1H, 2D5C9

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  WB analysis of HeLa using 67176-1-Ig (same clone as 67176-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67176-1-Ig (same clone as 67176-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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  WB analysis using 67176-1-Ig (same clone as 67176-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

  IP result of anti-DMC1 (IP:67176-1-Ig, 4ug; Detection:67176-1-Ig 1:1000) with mouse testis tissue lysate 2040 ug. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67176-1-Ig (DMC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67176-1-Ig (DMC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67176-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA DMC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DMC1 fusion protein Ag6951
• Vollständiger Name: DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)
• Berechnetes Molekulargewicht: 165 aa, 18 kDa, 38 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC035658
• Gene symbol: DMC1
• Gene ID (NCBI): 11144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.