Dystrophin Rekombinanter Antikörper

Dystrophin Rekombinant Antikörper für IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240567C4

Kat-Nr. : 83609-6-PBS

Synonyme

DMD, BMD, 240567C4

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  IHC staining of mouse skeletal muscle using 83609-6-RR (same clone as 83609-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83609-6-RR (Dystrophin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83609-6-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 83609-6-RR (same clone as 83609-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Dystrophin antibody (83609-6-RR, Clone: 240567C4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83609-6-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 83609-6-RR (same clone as 83609-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Dystrophin antibody (83609-6-RR, Clone: 240567C4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83609-6-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83609-6-RR (same clone as 83609-6-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug DMD Recombinant antibody (83609-6-RR, Clone:240567C4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83609-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83609-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83609-6-RR against Human Dystrophin were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83609-6-PBS bindet in IHC, IF-P, FC (Intra), ELISA Dystrophin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Dystrophin fusion protein Ag3408
• Vollständiger Name: dystrophin
• Berechnetes Molekulargewicht: 3685 aa, 427 kDa
• GenBank-Zugangsnummer: BC028720
• Gene symbol: Dystrophin
• Gene ID (NCBI): 1756
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.