DNA-RNA Hybrid Rekombinanter Antikörper

DNA-RNA Hybrid Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Dot blot, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

n/a

Anwendung

IHC, IF/ICC, FC (Intra), Dot blot, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250863A1

Kat-Nr. : 85855-1-PBS

Synonyme

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  IHC staining of human ovary tumor using 85855-1-RR (same clone as 85855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 85855-1-RR (DNA-RNA Hybrid antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85855-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 85855-1-RR (same clone as 85855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85855-1-RR (DNA-RNA Hybrid antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85855-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 85855-1-RR (same clone as 85855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85855-1-RR (DNA-RNA Hybrid antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85855-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85855-1-RR (same clone as 85855-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNA-RNA Hybrid antibody (85855-1-RR, Clone: 250863A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FBL antibody (66985-1-Ig, Clone: 3A9E8, red). This data was developed using the same antibody clone with 85855-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85855-1-RR (same clone as 85855-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNA-RNA Hybrid antibody (85855-1-RR, Clone: 250863A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FBL antibody (66985-1-Ig, Clone: 3A9E8, red). This data was developed using the same antibody clone with 85855-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 85855-1-RR (same clone as 85855-1-PBS)

  1x10^6 HEK-293T cells were intracellularly stained with 0.2 ug N/a DNA-RNA Hybrid Recombinant Antibody (85855-1-RR, Clone:250863A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 85855-1-PBS in a different storage buffer formulation.

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  Dot Blot experiment of / using 85855-1-RR (same clone as 85855-1-PBS)

  Dot blot analysis was used to confirm the specificity of 85855-1-RR DNA-RNA Hybrid antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (ng) spotted is indicated next to each row. This data was developed using the same antibody clone with 85855-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85855-1-PBS bindet in IHC, IF/ICC, FC (Intra), Dot blot, Indirect ELISA DNA-RNA Hybrid und zeigt Reaktivität mit n/a

• Getestete Reaktivität: n/a
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: unknow
• Vollständiger Name: DNA-RNA Hybrid
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA-RNA Hybrid antibodies are primarily used to study R-loops. Theoretically, they do not bind to single-stranded DNA, double-stranded DNA, or RNA. R-loops are DNA-RNA hybrid structures formed during transcription and are closely related to genome stability, DNA replication, DNA repair, and cancer development.