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DNA ligase III/LIG3 Rekombinanter Antikörper

DNA ligase III/LIG3 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242870C6

Kat-Nr. : 85409-4-PBS

Synonyme

LIG3, 242870C6, DNA ligase 3, DNA ligase III, EC:6.5.1.1

Filter:
  • Western Blot (WB) analysis of various lysates using DNA ligase III/LIG3 Recombinant antibody (85409-4-RR)

    WB analysis using 85409-4-RR (same clone as 85409-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85409-4-RR (LIG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85409-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85409-4-RR

    Affinity and Kinetic Characterization of 85409-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85409-4-RR against Human DNA ligase III/LIG3 were performed. The affinity constant is 3.04 nM.

Geprüfte Anwendungen

Produktinformation

85409-4-PBS bindet in WB, Indirect ELISA DNA ligase III/LIG3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen DNA ligase III/LIG3 fusion protein Ag24917
Vollständiger Name ligase III, DNA, ATP-dependent
Berechnetes Molekulargewicht 100-110 kDa
Beobachtetes Molekulargewicht 100 kDa
GenBank-ZugangsnummerBC068005
Gene symbol LIG3
Gene ID (NCBI) 3980
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA ligase III (DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.