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DNAH5 Polyklonaler Antikörper

DNAH5 Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31079-1-PBS

Synonyme

KIAA1603, HL1, Dynein heavy chain 5, axonemal, DNAHC5, Ciliary dynein heavy chain 5

Filter:
  • Immunohistochemistry (IHC) staining of human lung tissue using DNAH5 Polyclonal antibody (31079-1-AP)

    IHC staining of human lung using 31079-1-AP (same clone as 31079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 31079-1-AP (DNAH5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31079-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using DNAH5 Polyclonal antibody (31079-1-AP)

    IHC staining of mouse lung using 31079-1-AP (same clone as 31079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31079-1-AP (DNAH5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31079-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNAH5 Polyclonal antibody (31079-1-AP)

    IF Staining of HeLa using 31079-1-AP (same clone as 31079-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DNAH5 antibody (31079-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 31079-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DNAH5 Polyclonal antibody (31079-1-AP)

    IF Staining of HepG2 using 31079-1-AP (same clone as 31079-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DNAH5 antibody (31079-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31079-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

31079-1-PBS bindet in IHC, IF/ICC, Indirect ELISA DNAH5 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DNAH5 fusion protein Ag34647
Vollständiger Name dynein, axonemal, heavy chain 5
Berechnetes Molekulargewicht529 kDa
GenBank-ZugangsnummerNM_001369
Gene symbol DNAH5
Gene ID (NCBI) 1767
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dynein axonemal heavy chain 5 (DNAH5) is an axonemal heavy chain dynein, part of a microtubule-associated motor protein complex. DNAH5 is a large gene comprising 79 exons and one alternative first exon and encodes a heavy chain of the ODA. We have recently shown that recessive DNAH5 mutations are responsible for PCD characterized by ODA defects. DNAH5 mutations cause PCD and randomization of left-right asymmetry (PMID: 15750039).