DNAI1 Polyklonaler Antikörper

DNAI1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 12756-1-AP

Synonyme

PCD, ICS1, Dynein axonemal intermediate chain 1, CILD1

---

• 

  WB analysis of human brain using 12756-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12756-1-AP (DNAI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12756-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12756-1-AP (DNAI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12756-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12756-1-AP (DNAI1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IF Staining of hTERT-RPE1 using 12756-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DNAI1 antibody (12756-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, humanes Hirngewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 4 publications below

Produktinformation

12756-1-AP bindet in WB, IF/ICC, ELISA DNAI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAI1 fusion protein Ag3473
• Vollständiger Name: dynein, axonemal, intermediate chain 1
• Berechnetes Molekulargewicht: 699 aa, 79 kDa
• Beobachtetes Molekulargewicht: 80-85 kDa
• GenBank-Zugangsnummer: BC030583
• Gene symbol: DNAI1
• Gene ID (NCBI): 27019
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	J Cell Biol; Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm.; Authors - Fenglan Dong
human	IF	Elife; DNAH3 deficiency causes flagellar inner dynein arm loss and male infertility in humans and mice; Authors - Xiang Wang
human	IF	J Hum Genet; Novel variants in DNAH9 are present in two infertile patients with severe asthenospermia; Authors - Fei Yan
	IF	Nat Commun; Structure, interaction and nervous connectivity of beta cell primary cilia; Authors - Andreas Müller
mouse	WB,IF	EBioMedicine; CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men; Authors - Hui-Juan Jin