DNAI2 Polyklonaler Antikörper

DNAI2 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 17533-1-AP

Synonyme

CILD9, Dynein axonemal intermediate chain 2

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  WB analysis of mouse ovary using 17533-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17533-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 17533-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 17533-1-AP

  IP result of anti-DNAI2 (IP:17533-1-AP, 4ug; Detection:17533-1-AP 1:400) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of human heart using 17533-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17533-1-AP (DNAI2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse ovary using 17533-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17533-1-AP (DNAI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 17533-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17533-1-AP (DNAI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 17533-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DNAI2 antibody (17533-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Eierstockgewebe, HeLa-Zellen, Mauslungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: Maus-Eierstockgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 10 publications below
• IP: See 2 publications below

Produktinformation

17533-1-AP bindet in WB, IHC, IF-P, IP, ELISA DNAI2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAI2 fusion protein Ag11585
• Vollständiger Name: dynein, axonemal, intermediate chain 2
• Berechnetes Molekulargewicht: 593 aa, 67 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC039582
• Gene symbol: DNAI2
• Gene ID (NCBI): 64446
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IP,IF	Elife; ZMYND10 functions in a chaperone relay during axonemal dynein assembly.; Authors - Girish R Mali
mouse	IF	J Mol Cell Biol; Production of transgenic mice by random recombination of targeted genes in female germline stem cells.; Authors - Zhang Yong Y
rat	WB,IF	Front Cell Dev Biol; PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia; Authors - Tingting Zhang
mouse	WB	Development; CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility; Authors - Keisuke Shimada
mouse	WB	Exp Anim; The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum.; Authors - Haruhiko Miyata
human	WB	Ann Hum Genet; DNAH17 is associated with asthenozoospermia and multiple morphological abnormalities of sperm flagella.; Authors - Yanwei Sha