DNAI2 Polyklonaler Antikörper

DNAI2 Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17533-1-PBS

Synonyme

CILD9, Dynein axonemal intermediate chain 2

---

• 

  WB analysis of mouse ovary using 17533-1-AP (same clone as 17533-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 17533-1-AP (same clone as 17533-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 17533-1-AP (same clone as 17533-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse lung using 17533-1-AP (same clone as 17533-1-PBS)

  IP result of anti-DNAI2 (IP:17533-1-AP, 4ug; Detection:17533-1-AP 1:400) with mouse lung tissue lysate 4000ug. This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 17533-1-AP (same clone as 17533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 17533-1-AP (DNAI2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 17533-1-AP (same clone as 17533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17533-1-AP (DNAI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 17533-1-AP (same clone as 17533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17533-1-AP (DNAI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 17533-1-AP (same clone as 17533-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DNAI2 antibody (17533-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

17533-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA DNAI2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAI2 fusion protein Ag11585
• Vollständiger Name: dynein, axonemal, intermediate chain 2
• Berechnetes Molekulargewicht: 593 aa, 67 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC039582
• Gene symbol: DNAI2
• Gene ID (NCBI): 64446
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.