DNAJA1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DNAJA1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Rezensionen (1)

Kat-Nr. : 11713-1-AP

Synonyme

DJ 2, DjA1, DNAJ2, hDJ 2, HDJ2

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  WB analysis using 11713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11713-1-AP

  WB result of DNAJA1 antibody (11713-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJA1 transfected HeLa cells.

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  WB analysis of human liver using 11713-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11713-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11713-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 11713-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11713-1-AP (DNAJA1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11713-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DNAJA1 antibody (11713-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Lebergewebe, humanes Hodengewebe, Jurkat-Zellen, Mausnierengewebe, Mauslungengewebe, NIH/3T3-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 12 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

11713-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DNAJA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJA1 fusion protein Ag2284
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily A, member 1
• Berechnetes Molekulargewicht: 397 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC008182
• Gene symbol: DNAJA1
• Gene ID (NCBI): 3301
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.

Publikationen

Species	Application	Title
human	WB,IF	Nature; A cytosolic surveillance mechanism activates the mitochondrial UPR; Authors - F X Reymond Sutandy ; KD Validated
human	IF	Nat Cell Biol; Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition; Authors - Shan Lu
human	WB,IP	Nat Commun; Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.; Authors - Jiawei Zhang
human	WB	J Clin Invest; Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.; Authors - Yajuan Zhang ; KD Validated
mouse	WB	Mol Ther Nucleic Acids; Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction; Authors - Jie Pan
human	WB	Sci Rep; Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation.; Authors - Claudio Rodríguez-González ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Filip (Verified Customer) (10-28-2024)

I recommend this antibody. It does not recognize DNAJA2, which we run in parallel to DNAJA1.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000