DNAJA1 Monoklonaler Antikörper

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• KD/KO Validated

DNAJA1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B10A10

Kat-Nr. : 67184-1-PBS

Synonyme

HDJ2, hDJ 2, DNAJ2, DjA1, DJ 2

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  WB analysis using 67184-1-Ig (same clone as 67184-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67184-1-Ig (DNAJA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67184-1-Ig (same clone as 67184-1-PBS)

  WB result of DNAJA1 antibody (67184-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJA1 transfected HeLa cells. This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJA1 antibody (67184-1-Ig, Clone: 1B10A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67184-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DNAJA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DNAJA1 fusion protein Ag2284
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily A, member 1
• Berechnetes Molekulargewicht: 397 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC008182
• Gene symbol: DNAJA1
• Gene ID (NCBI): 3301
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Euglobulin-Präzipitation
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.