DNAJB1 Monoklonaler Antikörper

DNAJB1 Monoklonal Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E8E10

Kat-Nr. : 67422-1-PBS

Synonyme

1E8E10, DnaJ protein homolog 1, DNAJ1, hDj 1, Hdj1

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  WB analysis using 67422-1-Ig (same clone as 67422-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67422-1-Ig (DNAJB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67422-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67422-1-Ig (same clone as 67422-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNAJB1 antibody (67422-1-Ig, Clone: 1E8E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67422-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67422-1-Ig (same clone as 67422-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DNAJB1 (67422-1-Ig, Clone:1E8E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67422-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67422-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA DNAJB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DNAJB1 fusion protein Ag3830
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 1
• Berechnetes Molekulargewicht: 340 aa, 40 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC019827
• Gene symbol: DNAJB1
• Gene ID (NCBI): 3337
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.