DNAJB1 Rekombinanter Antikörper

DNAJB1 Rekombinant Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4K3

Kat-Nr. : 82676-1-PBS

Synonyme

hDj-1, Hdj1, hDj 1, DNAJ1, DnaJ protein homolog 1

---

• 

  WB analysis using 82676-1-RR (same clone as 82676-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82676-1-RR (DNAJB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse lung using 82676-1-RR (same clone as 82676-1-PBS)

  IP result of anti-DNAJB1 (IP:82676-1-RR, 4ug; Detection:82676-1-RR 1:5000) with mouse lung tissue lysate 1120 ug. This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82676-1-RR (DNAJB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82676-1-RR (DNAJB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82676-1-RR (same clone as 82676-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNAJB1 antibody (82676-1-RR, Clone: 4K3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82676-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82676-1-RR against Human DNAJB1 were performed. The affinity constant is 0.213 nM.

---

Geprüfte Anwendungen

Produktinformation

82676-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DNAJB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DNAJB1 fusion protein Ag3830
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 1
• Berechnetes Molekulargewicht: 340 aa, 40 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC019827
• Gene symbol: DNAJB1
• Gene ID (NCBI): 3337
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.